The carrier gas, helium, GC-MS analysis GC-MS analysis of bioactive compounds in stem bark ethanolic extract of C. mala-elengi was performed at Kerala Forest Research Institute, Thrissur District, Kerala (Casuga et al., 2016). 0.5ml of concentrated sulfuric acid was slowly added. . 2. by quantitative GC-MS analysis and phytochemical screening. gc/ms is a useful and dependable method for identifying complex plant extracts in an efficient and timely manner [53]. Methods Chemicals The method of GC-MS analysis of seven different plant extracts (4 plants and 3 combinations) described here was developed by Wang et al. Phytochemical screening of the sequential extracts of B.Cylindrica leaves revealed the presence of various bioactive components of which . The GC-MS analysis The GC-MS analysis of bioactive compounds from the different extracts of the leaves of B. luzonica was done using Agilent Technologies GC systems with GC-7890A/MS-5975C model (Agilent Technologies, Santa Clara, CA, USA) equipped with HP-5MS column (30 m in length 250 m in diameter 0.25 m in thickness of film). The GC-MS analysis revealed the 18 chemical compounds. 59. GC/MS Analysis The chemical constituents identified by the GC/MS analysis of the methanolic plant extract of Mentha spicata (L.) was enumerated along with their molecular formula, retention time, and peak area (Table 3). identied. GC-MS chromatogram analysis of methanol leaf extract of mangosteen. GC-MS procedure was performed by utilizing GC Shimadzu QP2010 framework and gas chromatograph interfaced to a Mass Spectrometer (GC-MS) outfitted with Elite-1 combined silica hair like segment. ZB 5- MS capillary standard Non-Polar column was used. . mixed aqueous extract of D.gangeticum root is efficient in protecting the heart from ischemia reperfusion induced injury in diabetic rats 31 and the extracts of D.gangeticum have antimicrobial potential 32. The dry extracts were saponified with 2 mol/L KOH (dissolved in 50% ethanol) under reflux for 1.5 h. After cooling, the obtained extracts were separated in triplicate by liquid-liquid extraction with n-hexane. Further, the. GC -MS Analysis Gas chromatography Mass spectroscopy analysis of ethanolic extract was performed using Shimadzu GC-MS Model number: QP2010S equipped with Column - ELITE-5MS (30 meter length, 0.25 mm ID, and 0.25 m thicknesses). The GCMS analysis of ethanol extracts of both OPS and WPS samples were performed in GC-MS analysis of the extract was performed using a Perkin . Oven temperature was programmed to 60 oC for 10 min, and then increased at 20 oC/min to 230 oC and held at 250 oC for 10 min. GC MS analysis GC-MS analysis was carried out on a Shimadzu GCMSQP2010 Medicinal plants are rich in various bioactive compounds such as alkaloids, steroids, flavonoids, glycosides, The preliminary phytochemical screening reveals the . Kale MV. Phytochemicals such as . 1L of the supernatant was injected into the GC and run for 30 minutes. (2005). For ve-hicle control, 10l of ethanol was added in triplicate . The results concluded that the callus have more . In the present work, compounds are identified by GC-MS of methanol extract of P.viscida and D. gangeticum, antibacterial GC-MS Analysis of Ethanolic Extract of Alternanthera Philoxeroides and Alternanthera Bettzickiana from India U. Arul Pamila1 and S. Karpagam2 1PhD Research Scholar, 2Assistant Professor, Department of Botany, Queen Mary's College (A), Chennai-600 004. Results and Discussion The scientific validation and extraction of plant The major constituents were found to be 2-methylheptane (3.48%), cis-1,3-dimethylcyclohexane International Journal of Research in Applied Natural and Social Science 2013; 1(3):49-58. Datura stramonium is known as medicinal plant worldwide. Flow rate of carrier gas was 1.2 ml/min. Leucophloea with the NIST & WILEY libraries, major Alkaloids are used as anaesthetic agents and are found bioactive chemical constituents found in the extract in medicinal plants [8]. It shows the presence of nearly forty constituents which includes flavonoids, 2. The sample volume was 4.0 L. Table 1. Gas chromatography-Mass spectrometry Analysis: For quantitation (area %), the GC analyses . 9, 12-Octadecadienoic acid (ZZ) was the most potent bioactive compound present in three different extracts of both B. hispida and C . Different extracts of targeted seeds showed different biologically active compounds that have different pharmacological potentialities. Figure 2 shows the mass spectra of methanolic extract of Moringa oleifera Fig 1 Shows GCMS chromatogram of methanolic extract of Moringa oleifera The plant powder (10 gm) was taken and mixed with ethanol (150 ml).The mixture was boiled until and it was reduced . The program settings were capillary column TG-5MS AMINE (30 m 0.25 mm; film The present investigation has helped to identify seventy five (75) bioactive chemical constituents from leaf, root in vivo and in vitro leaf callus methanolic extracts of Momordica cymbalaria by using GC-MS technique. The present study is the comparative GC-MS analysis of an edible plant Alternanthera GC-MS analysis: The Agilent 5975C Series GC/MS used in the analysis employed a fused silica column packed with DB-WAX (100 % dimethyl polysiloxane, 30 m 0.25 mm ID 2.5 m ). E ach of the extracts was analyzed by gas chromatography-mass spectrometry. GC-MS Analysis of Ethyl Acetate Extract of Whole plant of Rostellularia diffusa. GC-MS Analysis The GC-MS analysis of A.paniculata extracts was carried out on a Thermo GC 1300 and TSQ 8000 Triple Quadrupole GC- MS system with auto sampler Al 1310 at USIC Department of University of Rajasthan, Jaipur. 30 m length). The medicinally important plants Cissus xavierensis, C. quadrangularis var. GC MS Analysis of Bioactive Compounds Present in Ethanol Extract of Combretum Hispidum (Laws) (Combretaceae) Leaves International Journal of Trend in Scientific Research and Development (IJTSRD) Volume 4 Issue 5, August 2020 Available Online: www.ijtsrd.com e-ISSN: 2456 -6470 GC-MS-olfactometric characterization of the most aroma-active components in a representative aromatic extract from Iranian saffron (Crocus sativus L.) Asghar Amanpour 2015, Food Chemistry Gas chromatography-mass spectroscopy (GC-MS) is a combined analytical technique used to determine and identify compounds present in a plant sample [ 10 ]. Materials and Methods 2.1. subtilis 168 Isolated from . The present research was designated to investigate the methanolic extract used by GC-MS Technique (Thermo GC-Trace ultra version 5.0, Fisher, USA). gc-ms chromatogram of the ethanol leaf extract of cassia alata (figure 2) showed 13 peaks indicating the presence of thirteen compounds with the retention time range between 2.72 and 34.54 (figure 2).the active principles in the methanol leaf extract of cassia alata were confirmed based on retention time (rt), molecular formula, molecular weight Int J Drug Dev Res. spot obtained (Appendix 1) was used for GC- MS analysis. Students utilized QuEChERS to analyze spiked and incurred pesticide residues in several types of plant foods and applied GC-MS/SIM for the simultaneous quantitation . Researchers have explored extracts of plants including its derivatives for . Plant materials preparations. Carrier gas: He, Flow: 1.0 The screening of plant extracts is a new approach to find therapeutically active compounds in various plant species [1, 5]. GC-MS plays an essential role in the phytochemical analysis and chemotaxonomic studies of medicinal plants containing biologically active components [ 11 ]. This plant's leaves juice from its roots is used against asthma and whooping cough Corresponding Author: A Manimaaran P.G. LC-MS/MS comparison of the 4 plant extracts imply that Y.aloifolia variegata L extract was the richest, which was apparent through its superior biological activity. GC-MS Analysis GC-MS analysis was carried out on the ethanolic extract of Canthium parviflorum and 22 compounds were identified. Collection of Plant and Preparation of Extract Senna alata plant leaves were obtained from Oba Ile area of Akure and confirmed by Herb sellers at Oja Oba market in Akure. GC- MS, with the use of internal standards, provides a multidimensional compound identification and quantification of the sample (Skoog et al., 2007). Plant extract with code Plant extract code Explanation R.D But Rosa damascena extract in butanol R.D Ethyl acet. The volatile compounds in the sample were analyzed by Gas Chromatography Mass Spectrometer (GC/MS) on an Agilent Phytochemical screening of Trichosanthes anguina L. (Snake gourd) root was done through GC-MS . The GC/MS analysis of Mentha spicata (L.) revealed the presence of 48 compounds identified in the methanolic extract. GCMS is one of the most precise methods to identify various secondary metabolites present in the plant extract The ethanolic extract of E. crassipes was analyzed by GCMS to detect various compounds with the help of NIST library. Pharmacog J, 9(1), pp. Dimension 30Mts, ID: 0.25mm, Film: 0.25 m. The 1.0 ml/min volume of methanolic extract sample injected into the instrument was GC-MS analysis GC-MS analysis was carried out in a GC-MS: HP5890 fitted with a 1.4 m . Several of these compounds are documented to . Preparation of plant extract Fresh plant was shade dried at room temperature for 10 days and powdered coarsely using electric blender. The GC-MS analysis of P. suberosa stem bark revealed the presence of twelve compounds (phytochemical constituents) that could contribute to the medicinal quality of the plant. GC-MS analysis The GC-MS analysis of the extracts was performed using a Perkin Elmer GC-MS (Model Perkin Elmer Clarus 600) equipped with an Generally it is used as a traditional medicine due to its medicinal values. Analysis was Rosa damascena extract in ethyl acetate GC-MS analysis GC/MS was performed with model 'Agilent GC-MS triple quad 7000 GC 7890A'. The Gas Chromatography-Mass Spectrometry (GCMS) analysis of the extracts was performed using a GC-MS (Model; QP 2010 series, Shimadzu, Tokyo, Japan) fitted withVF-5ms fused silica capillary column of 30 m length, 0.25 mm diameter, and film thickness: 0.25 mm. Myristica fragrans (f. Myristicaceae) seeds are better known as a spice, but their chemical compounds may have a pharmacological effect. 10mg of the dried extract powder was encapsulated in 100 mg of KBr pellet, in order to prepare translucent sample disc. We optimised the analytical and experimental bottlenecks that captured free, ester, glycoside and wall-bound phenolics after acid or alkali treatments of the tissue extracts and subsequent GC-MS analysis. GC-MS analysis of extract of leaves Solanum torvum revealed the exsistance of 3-n-hexylthiolane, S,S-dioxide (49.407%) was found as the . Antimicrobial Activity and GC-MS Based Analysis of the Extract of Bacillus subtilis subsp. Selvamangai C, Bhaskar A. GC-MS analysis of Phytocomponents in the Methanolic extract of Eupatorium triplinerve. The objective of this present work is to identify the. The column used was Perkin Elmer Elite - 5 capillary column measuring 30m 0.25mm with a film thickness of 0.25mm composed of 95% Dimethyl polysiloxane. extracts by GC-MS analysis. [23] [24]. The solution was boiled in a water bath and then incubated at 50-70C for an hour followed by filtering from What man paper [9]. GC-MS analysis of the active methanol extract of L. camara was carried out by using the GC-MS instrument (Model GCMS-QP2010 Ultra, Shimadzu Co., Japan) equipped with a capillary column DB-1 (0.25 m film 0.25 mm i.d. International Journal of Advanced Scientific Research and Management , Volume 4 Issue 5, May 2019 www.ijasrm.com ISSN 2455-6378 GC-MS Analysis For Identification Of Bioactive Compounds In Crude Methanolic Extracts Of Leaves Of Origanum Majorana L. Plant Khushbo Bhardwaj1* and Widhi Dubey2 1 Faculty of Science, JECRC University, Jaipur, 303905, India 2 Faculty of Science, JECRC University . Fig. subtilis 168 Isolated from a River . The yield of their composition of extracts and essential oils differs due to different methodologies. The prepared extract is also analysed by gas chromatography-mass spectrometry to identify and characterize the chemical compounds present in the extract. Robust methodologies aimed at efficient extraction of plant phenolics, their qualitative and quantitative analysis is desired. Investigations on the Availability of Bioactive Compounds in . subjected to GCMS analysis which was prepared by ultrasonication method. GC-MS analysis of phytocomponents on whole plant extracts Adiantum capillus-veneris L. - a potential folklore medicinal plant. the dried extract was stored in a deep freezer for further use. Fig. 0.02 of extract was dissolved in 1ml of glacial acetic acid and 1-2 drops of ferric chloride solution was added. One study has stated that the mangosteen leaf extract was very effective against both, the Gram positive and the Gram negative bacteria, specifically those that were linked with skin infections like acne, diarrhoea, tuber- culosis etc. 4: Type of Chemical compound in Flowers extract of C. indica plant. ISBN 978--521-56123-5. This family is one of the largest family in plant kingdom comprising of highest number of edible plant species. A small portion of the dry extracts was screened for the presence of chemical constituents like alkaloids, carbohydrates, glycosides, phenols, saponins, phytosterols, flavanoids, proteins and terpenoids [8, 9]. and Research Department of Advanced Zoology and Biotechnology, Government Arts College for Men, Nandanam, Chennai, Tamil Nadu, India Antioxidant, antimicrobial activities and GCMS analysis of fruit extract of Solanum nigrum L. 70-72. Conclusion: From this work, it can be concluded that the species contain eective phytochemical compounds, needs further research on toxicological aspects to develop a safe drug. GC was performed using a Varian GC/MS 4000 equipped with a VF-5ms MS capillary column (30 m x 0.25 mm i.d., 0.25 m) (Agilent Technologies 7890A GC System, G3171A, USA). The cleaned extract was solvent exchanged into toluene and analyzed for pesticides by capillary gas chromatography-mass spectrometry in selective ion monitoring mode (GC-MS/SIM). GC-MS analysis offers . Ebenezer T, Kadamban D, Shekhawat MS, Justin Packia J. GC-MS Analysis Gas Chromatography-Mass Spectrometry (GC-MS) is an analytical method that combines the features of Gas- Chromatography (GC) and Mass Spectrometry (MS). . n-Hexane extract of root of Rauvolfia serpentina was analyzed through GC-MS. The GC-MS analysis of R. humilis stem revealed the presence of various compounds which have been listed in table 1 and figure 1. resulting extract was dried using a water bath to get semisolid. GC-MS analysis: The ethyl acetate plant extract of . them involve the use of plant extracts 1. The present study was planned to investigate the number of constituents present in chloroform extract of whole plant of Calotropis gigantean Linn. A brown ring at the interface indicated a deoxy sugar characteristic of cardenolides. the objective of this paper was to review and investigate the phytochemical constituents with gc-ms analysis and to establish the antibacterial property of the plant extract against the bacterial pathogens, escherichia coli and klebsiella pneumonia (which are proven causal organisms for all forms of urinary tract infections (uti) and also liver from the 2-fold diluted plant extract stocks ranging from .4g/ml to 3g/ml were added in triplicate wells. PDF | On Aug 25, 2022, Olasinbo Olumuyiwa Balogun and others published Antimicrobial Activity and GC-MS Based Analysis of the Extract of Bacillus subtilis subsp. 58. Identification was based on the molecular structure, molecular mass and calculated fragments. Furthermore, GC MS analysis of the extract revealed the presence of 65 bioactive compounds. The GC-MS analysis was done using the instrument GC-MS SHIMADZU QP2010 with GCMS solution version 2.53 software. The GCMS analysis of C. bicolor plant aqueous extract exhibited the presence of 98 compounds with 10 known bioactive uses. In this study we performed GCMS, to analysis methanolic plant extracts of Momordica cymbalaria Fenzl. 2.4 GC-MS analysis The phytochemicals of Kayea assamica leaf was analyzed by GC-MS. GC-MS is one of the best techniques to identify the constituents in plants. Electron ionization system was used; details of GC programme were given in Table 1. 20. It was . The aim of this study was to evaluate an excipient materialmagnesium aluminometasilicateand to determine its influence on the qualitative . The GC-MS analysis shows different peaks with low and high molecular weight determining the presence of 41 phytochemical compounds.The Phytochemical compounds in the ethanolic extract ofLawsonia inermishave been screened by using GC-MS analysis. 2012;4:148-53. and 7.35% saponins in plant sample. Then plant root extract (CBE) was prepared by mixing 1-5% of plant root powder with deionized water in a 250 ml of (Borosil, India) conical flask. CAS.N0 Retention Peak Molecular Structure Compound No. GC-MS analysis GC-MS analysis were carried out . GC-MS analysis of the whole plant ethanol extract of P. chinense was performed using a Perkin-Elmer GC Clarus 500 system comprising an AOC-20i auto-sampler and a gas chromatograph interfaced to a mass spectrometer (GC-MS). The electron ionization device used for GC-MS detection had a 70 eV ionisation energy. The presence of these compounds may proceed to find out various therapeutic activities.